May 1998, Volume 11, Number 5 Pages 404-412
DOI: 10.1094/MPMI.1998.11.5.404
Magnaporthe grisea Pathogenicity Genes Obtained Through Insertional Mutagenesis James A. Sweigard, 1 Anne M. Carroll, 1 Leonard Farrall, 2 Forrest G. Chumley, 3 and Barbara Valent2 1Central Research and Development Department, and 2DuPont Agricultural Products, E. I. du Pont de Nemours and Co., Inc., P.O. Box 80402, Wilmington, DE 19880-0402 U.S.A.; and 3DuPont Agricultural Products, Barley Mill Plaza, P.O. Box 80037 Wilmington, DE 19880-0037 U.S.A.  Open Access.
We have initiated a mutational analysis of pathogenicity in the rice blast fungus, Magnaporthe grisea, in which hygromycin-resistant transformants, most generated by restriction enzyme-mediated integration (REMI), were screened for the ability to infect plants. A rapid primary infection assay facilitated screening of 5,538 transformants. Twenty-seven mutants were obtained that showed a reproducible pathogenicity defect, and 18 of these contained mutations that cosegregated with the hygromycin resistance marker. Analysis of eight mutants has resulted in the cloning of seven PTH genes that play a role in pathogenicity on barley, weeping lovegrass, and rice. Two independent mutants identified the same gene, PTH2, suggesting nonrandom insertion of the transforming DNA. These first 7 cloned PTH genes are described. Cited byUnder pressure: investigating the biology of plant infection by Magnaporthe oryzaeNature Reviews Microbiology Apr 2009, Volume 7, Number 3: 185-195 CrossRef Discovery of Pathogenicity Genes in the Crucifer Anthracnose Fungus Colletotrichum higginsianum, Using Random Insertional MutagenesisMolecular Plant-Microbe Interactions Feb 2009, Volume 22, Number 2: 143-156 Abstract
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