January 2001, Volume 14, Number 1 Pages 98-103
DOI: 10.1094/MPMI.2001.14.1.98
Research Note Construction of a Derivative of Agrobacterium tumefaciens C58 That Does Not Mutate to Tetracycline Resistance Zhao-Qing Luo, 1 Thomas E. Clemente, 3 and Stephen K. Farrand1,2 Departments of 1Crop Sciences; and 2Microbiology, University of Illinois at Urbana-Champaign, Urbana 61801, U.S.A.; 3Center for Biotechnology, University of Nebraska, Lincoln 68588, U.S.A.  Open Access.
Agrobacterium tumefaciens C58 mutates to tetracycline resistance at high frequency, complicating the use of many broad-host-range cloning and binary vectors that code for resistance to this antibiotic as the selection marker. Such mutations are associated with a resistant gene unit, tetC58, that is present in the genome of this strain. By deleting the tetC58 locus, we constructed NTL4, a derivative of C58 that no longer mutates to tetracycline resistance. The deletion had no detectable effect on genetic or physiological traits of NTL4 or on the ability of this strain to transform plants. Additional keywords: genetic engineering. Cited byIdentification of amino acid residues of the pheromone-binding domain of the transcription factor TraR that are required for positive controlMolecular Microbiology Sep 2009 CrossRef Dimerization of the quorum-sensing transcription factor TraR enhances resistance to cytoplasmic proteolysisMolecular Microbiology Aug 2009, Volume 73, Number 1: 32-42 CrossRef Characteristics of the LrhA subfamily of transcriptional regulators from Sinorhizobium melilotiActa Biochimica et Biophysica Sinica Mar 2008, Volume 40, Number 2: 166-173 CrossRef Pseudomonas corrugata contains a conserved N-acyl homoserine lactone quorum sensing system; its role in tomato pathogenicity and tobacco hypersensitivity responseFEMS Microbiology Ecology Sep 2007, Volume 61, Number 2: 222-234 CrossRef The quorum-sensing transcription factor TraR decodes its DNA binding site by direct contacts with DNA bases and by detection of DNA flexibilityMolecular Microbiology May 2007, Volume 64, Number 1: 245-256 CrossRef Co-expression of the borage Δ6 desaturase and the Arabidopsis Δ15 desaturase results in high accumulation of stearidonic acid in the seeds of transgenic soybeanPlanta Oct 2006, Volume 224, Number 5: 1050-1057 CrossRef Rapid and reproducible Agrobacterium-mediated transformation of sorghumPlant Cell Reports Aug 2006, Volume 25, Number 8: 784-791 CrossRef Long- and Short-Chain Plant-Produced Bacterial N-Acyl-Homoserine Lactones Become Components of Phyllosphere, Rhizosphere, and SoilMolecular Plant-Microbe Interactions Mar 2006, Volume 19, Number 3: 227-239 Abstract
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