March 2001, Volume 14, Number 3
Pages 422-425
DOI: 10.1094/MPMI.2001.14.3.422
Research Note
A Bacterial Artificial Chromosome Library of Lotus japonicus Constructed in an Agrobacterium tumefaciens-Transformable Vector
Artem E. Men,
1,2 Khalid Meksem,
3 My Abdelmajid Kassem,
3 Dasharath Lohar,
2 Jiri Stiller,
1,2 David Lightfoot,
3 and
Peter M. Gresshoff1 1Department of Botany, University of Queensland, Brisbane, QLD 4072, Australia; 2Plant Molecular Genetics, University of Tennessee, Knoxville 37996-4500, U.S.A.; 3Department of Plant Soil and General Agriculture, Southern Illinois University, Carbondale 62901-4415, U.S.A.
Open Access.
We constructed a BAC library of the model legume Lotus japonicus with a 6-to 7-fold genome coverage. We used vector PCLD04541, which allows direct plant transformation by BACs. The average insert size is 94 kb. Clones were stable in Escherichia coli and Agrobacterium tumefaciens.
Additional keywords: Arabidopsis, functional genomics, map-based cloning, symbiosis.
