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SPECIAL REPORT

Development of an Inoculation Technique for Rapidly Evaluating Maize Inbred Lines for Resistance to Stalk Rot caused by Fusarium spp. in the Field

    Affiliations
    Authors and Affiliations
    • Wanyi Jiang1
    • Wenbo Han1
    • Ruiting Wang1
    • Yonggang Li1 †
    • Guanghui Hu2
    • Jianfei Yang2
    • Dan Jiang1
    • Wenhao Han1
    • Mingquan Wang2
    • Guoliang Li2
    1. 1Agricultural College, Northeast Agricultural University, Harbin 150030, P. R. China
    2. 2Institute of Maize Research, Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, Heilongjiang, China
    Published Online:29 Oct 2021https://doi.org/10.1094/PDIS-09-20-2016-SR

    Abstract

    Maize (Zea mays L.) stalk rot, caused primarily by the soilborne fungal pathogen Fusarium spp., reduces maize quality and yield worldwide. This study was undertaken to develop and utilize a rapid continuous injection inoculation technique to evaluate maize inbred lines for resistance to Fusarium spp. under field conditions, which could facilitate the identification and development of new sources of host resistance to manage the disease. Continuous injection inoculation is a rapid, stable, and simple method that can evaluate the resistance of maize inbred lines to Fusarium stalk rot (FSR) within 20 days. To verify the feasibility and reliability of the inoculation method, Fusarium graminearum, F. proliferatum, and F. subglutinans were isolated, identified, and inoculated into maize at the six-leaf stage (V6) by a veterinary adjustable bottle continuous vaccination syringe. Our results showed that, of a total of 97 inbred maize lines, 6 (6.2%) showed high resistance to maize stalk rot, 20 showed resistance (20.6%), 32 were susceptible (33.0%), and 39 were very susceptible (40.2%). Based on simple sequence repeat markers, an analysis of molecular variance indicated a significant correlation between population of the inbred maize line and resistance to FSR (P = 0.001). Overall, this study provided a systematic, rapid, stable, and simple identification method for maize inbred lines resistant to FSR in the field. At the same time, this method was also suitable for genetic diversity analysis of maize inbred lines resistant to FSR.

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