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OPENOpen Access licenseCharacterization of the Sweet Cherry Isolate of Plum Pox Potyvirus
- A. Crescenzi
- L. d'Aquino
- S. Comes
- M. Nuzzaci
- P. Piazzolla , Dipartimento di Biologia, Difesa e Biotecnologie Agro Forestali, Università degli Studi della Basilicata, Potenza, Italy
- D. Boscia , Dipartimento di Protezione delle Piante, Università degli Studi and Centro di Studio sui Virus e le Virosi delle Colture Mediterranee, Bari, Italy
- A. Hadidi , National Germplasm Resources Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705
Abstract
An isolate of plum pox potyvirus from sweet cherry (PPV-SwC) in southern Italy was investigated. The isolate was mechanically or graft transmissible to different Prunus and Nicotiana spp. but not to Chenopodium spp. It was transmitted also by Aphis fabae and Myzus persicae in a nonpersistent manner. Restriction fragment length polymorphism analysis indicated and nucleotide sequencing confirmed that the isolate lacks AluI and RsaI sites in the C-terminal region of the coat protein (CP) gene. Western immunoblot results showed that the PPV-SwC CP has an electrophoretic mobility similar to that of strain PPV-D and faster than that of strain PPV-M. Double-antibody sandwich indirect enzyme-linked immunosorbent assay of the CP showed that PPV-SwC, although reacting with universal monoclonal antibodies to PPV, failed to react with antibodies specific to strains M and D. Results indicate that PPV-SwC is different from conventional strains of PPV but closely related to the sour cherry isolate of PPV from Moldova.
