RESEARCH

Department of Plant, Soil, and Microbial Sciences, Michigan State University, East Lansing, MI 48824

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Jan M. Byrne

Department of Plant, Soil, and Microbial Sciences, Michigan State University, East Lansing, MI 48824

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Monique L. Sakalidis

http://orcid.org/0000-0001-6251-7724

Department of Plant, Soil, and Microbial Sciences, Michigan State University, East Lansing, MI 48824

Department of Forestry, Michigan State University, East Lansing, MI 48824

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Timothy D. Miles

^†Corresponding authors: T. D. Miles;

E-mail Address: milesti2@msu.edu

, and M. K. Hausbeck;

E-mail Address: hausbec1@msu.edu

http://orcid.org/0000-0002-7484-9360

Department of Plant, Soil, and Microbial Sciences, Michigan State University, East Lansing, MI 48824

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, and

Mary K. Hausbeck

^†Corresponding authors: T. D. Miles;

E-mail Address: milesti2@msu.edu

, and M. K. Hausbeck;

E-mail Address: hausbec1@msu.edu

Department of Plant, Soil, and Microbial Sciences, Michigan State University, East Lansing, MI 48824

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Affiliations

Authors and Affiliations

Douglas S. Higgins¹
Ross J. Hatlen¹
Jan M. Byrne¹
Monique L. Sakalidis¹²
Timothy D. Miles¹^†
Mary K. Hausbeck¹^†

¹Department of Plant, Soil, and Microbial Sciences, Michigan State University, East Lansing, MI 48824
²Department of Forestry, Michigan State University, East Lansing, MI 48824

Published Online:26 Jan 2021https://doi.org/10.1094/PDIS-05-20-0924-RE

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Abstract

Michigan’s hop acreage ranks fourth nationally, but the state’s growers contend with unique disease challenges resulting from frequent rainfall and high humidity. In August 2018, a Michigan hop grower reported necrosis and blighting of foliage and shattering of cones resulting in yield loss. Irregular-shaped lesions developed on leaves, surrounded by a halo of chlorotic tissue, and cone bracts became brown. Pycnidia were observed in symptomatic tissue. The goal of this study was to identify and characterize the causal agent of symptoms in leaf and cone tissue. In symptomatic leaves, 15 of 19 isolates recovered had 96.4% internal transcribed spacer rDNA (ITSrDNA) homology with Diaporthe nomurai. Bayesian and maximum likelihood analyses were performed on a subset of isolates using ITSrDNA, histone H3, beta-tubulin, and elongation factor 1 alpha. Bootstrap and posterior probabilities supported a unique cluster of Diaporthe sp. 1-MI isolates most closely related to the Diaporthe arecae species complex, Diaporthe hongkongensis, and Diaporthe multigutullata. Diaporthe sp. 1-MI was pathogenic in detached leaf and whole plant assays. Single-spore isolates from pycnidia originating from cones and leaves shared 100% ITSrDNA homology with Diaporthe sp. 1-MI obtained from the lesion margins of leaves collected in 2018. The distribution of Diaporthe sp. 1-MI was widespread among 347 cones collected from 15 Michigan hop yards and accounted for >38% of fungi recovered from cones in three hop yards. Diaporthe sp. 1-MI causing halo and cone blight presents a new disease management challenge for Michigan hop growers.

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Vol. 105, No. 4
April 2021

ISSN:0191-2917
e-ISSN:1943-7692

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Leaf symptom of field-grown ‘Hayward’ kiwifruit plant infected with Actinidia virus (L. Zhao et al.). Photo credit: L. Zhao. Leaf spot of Italian ryegrass naturally infected by Alternaria alternata (X. K. Wei et al.). Photo credit: L. H. Xue. Leaf of Ficus carica displaying symptoms of fig mosaic disease (S. Preising et al.). Photo credit: W. L. da Silva.

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Issue Date: 21 Apr 2021
Published: 26 Jan 2021
First Look: 25 Aug 2020
Accepted: 18 Aug 2020

Pages: 859-872

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Funding

MSU Project GREEEN
Grant/Award Number: GR17-022
Grant/Award Number: GR19-016

USDA Specialty Crop Block Grant Program
Grant/Award Number: Michigan-791AgDSC1805

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The author(s) declare no conflict of interest.

Etiology of Halo Blight in Michigan Hopyards

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