Short Communication

Comparative Genomics of Barley-Infecting Xanthomonas translucens Shows Overall Genetic Similarity but Globally Distributed Virulence Factor Diversity

    Affiliations
    Authors and Affiliations
    • Nathaniel Heiden1 2
    • Veronica Roman-Reyna1 2
    • Rebecca D. Curland3
    • Ruth Dill-Macky3
    • Jonathan M. Jacobs1 2
    1. 1Department of Plant Pathology, The Ohio State University, Columbus, OH 43210
    2. 2Infectious Diseases Institute, The Ohio State University, Columbus, OH 43210
    3. 3Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108

    Xanthomonas translucens pv. translucens (Xtt) is a global barley patho-gen and a concern for resistance breeding and regulation. Long-read whole genome sequences allow in-depth understanding of pathogen diversity. We have completed long-read PacBio sequencing of two Minnesotan Xtt strains and an in-depth analysis of available Xtt genomes. We found that average nucleotide identity (ANI)-based approaches organize Xtt strains different from the previous standard multilocus sequencing analysis approach. According to ANI, Xtt forms a separate clade from X. translucens pv. undulosa and consists of three main groups which are represented on multiple continents. Some virulence factors, such as 17 Type III-secreted effectors, are highly conserved and offer potential targets for the elicitation of broad resistance. However, there is a high degree of variation in virulence factors, meaning that germplasm should be screened for resistance with a diverse panel of Xtt.

    Literature Cited