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OPENOpen Access licenseMutagenesis of all Eight avr Genes in Xanthomonas campestris pv. campestris Had No Detected Effect on Pathogenicity, But One avr Gene Affected Race Specificity
AbstractAbstract
Suppression subtractive hybridization (SSH) was used to identify genes present in the systemic crucifer black rot pathogen Xanthomonas campestris pv. campestris 528T but missing from the nonsystemic crucifer leaf spot pathogen, X. campestris pv. ...- OPENOpen Access license
Production of Antimicrobial Defensin in Nicotiana benthamiana with a Potato Virus X Vector
AbstractAbstract
A recombinant plasmid, pTXS.TH, was constructed to express the gene-encoding wasabi (Wasabia japonica) defensin with the potato virus X (PVX) vector. pTXS.TH allows the expression of defensin in the host Nicotiana benthamiana, and the defensin protein WT1 ... - OPENOpen Access license
Analysis of 16S rDNA Sequences from Citrus Huanglongbing Bacteria Reveal a Different “Ca. Liberibacter” Strain Associated with Citrus Disease in São Paulo
AbstractAbstract
ABSTRACTCitrus huanglongbing (HLB, ex greening) is one of the most serious and destructive citrus diseases in the world. It is caused by a phloem-limited and nonculturable bacterium, “Candidatus Liberibacter”. The disease occurs in some Asian and African ... Novel Primer Sets for Rapid Detection of Zymoseptoria tritici in Wheat
AbstractAbstract
Zymoseptoria tritici is a fungal pathogen causing losses in wheat yields. Here, we present new primer sets for species-specific identification of this microorganism in wheat leaf samples using conventional PCR. Primer sets were validated in silico using ...Susceptibility of Garden Plants to Phytophthora Root Rot
AbstractAbstract
Phytophthora root rot (PRR) is a serious disease of horticultural, forest, and ornamental plant species caused by species of the oomycete genus Phytophthora. Their wide host range makes the choice of resistant plants in the management of the disease ...Illumina Sequencing of 18S/16S rRNA Reveals Microbial Community Composition, Diversity, and Potential Pathogens in 17 Turfgrass Seeds
AbstractAbstract
The increasing need for turfgrass seeds is coupled with the high risk of dangerous microbial pathogens being transmitted through the domestic and international trade of seeds. Concerns continue to be raised about seed safety and quality. Here, we show ...Susceptibility of Garden Trees and Shrubs to Armillaria Root Rot
AbstractAbstract
Armillaria root rot (ARR) is a serious disease of woody plants caused by several species of Armillaria. Armillaria isolates from diagnostic samples received in 2017 were identified by genus- and species-specific PCR and compared with isolates from an ...Monitoring Benzimidazole Resistance in Phyllosticta citricarpa Using a Molecular Assay Targeting Mutations in Codons 198 and 200 of the β-Tubulin Gene
AbstractAbstract
Citrus black spot (CBS), caused by Phyllosticta citricarpa, is an economically important disease, which is effectively controlled by repeated fungicide applications to protect fruit from infection. Systemic fungicides such as benzimidazoles are widely ...Rapid Detection of the Recently Identified Turfgrass Pathogen Magnaporthiopsis meyeri-festucae Using Recombinase Polymerase Amplification
AbstractAbstract
Magnaporthiopsis meyeri-festucae is a recently identified root-infecting pathogen of fine fescue (Festuca spp.) turfgrasses. Although it is phylogenetically similar to other root-infecting turfgrass pathogens such as M. poae, management of M. meyeri-...- OPENOpen Access license
Coffee Leaf Scorch Bacterium: Axenic Culture, Pathogenicity, and Comparison with Xylella fastidiosa of Citrus
AbstractAbstract
ABSTRACTSymptoms of coffee leaf scorch (CLS) appear on young flushes of field plants as large marginal and apical scorched areas on recently mature leaves. Affected leaves drop, shoot growth is stunted, and apical leaves are small and chlorotic. Symptoms ... - OPENOpen Access license
Relative Incidence of Erwinia carotovora subsp. atroseptica in Stolon End and Peridermal Tissue of Potato Tubers in Canada
AbstractAbstract
ABSTRACTAn enrichment enzyme-linked immunosorbent assay (ELISA) using monoclonal antibody 4F6, which is specific for the lipopolysaccharide antigen of serogroup I strains of Erwinia carotovora subsp. atroseptica, was used to detect the blackleg pathogen ... Phylogenetic Analysis and Genetic Diversity of Phytophthora palmivora Causing Black Pod Disease of Cocoa in Malaysia
AbstractAbstract
Black pod, caused by Phytophthora spp., occurs worldwide and is a major problem for cocoa farmers in Malaysia. Limited studies addressed causal agents of black pod disease of cocoa in Malaysia as well as their genetic diversity. Therefore, this study was ...- OPENOpen Access license
Morphological and Molecular Characterization of Globodera Populations from Oregon and Idaho
AbstractAbstract
ABSTRACTAn unusual population of cyst nematode was found in soils collected from a Powell Butte, OR field with a cropping history including potato, wheat, other crops, and significant weed presence. These nematodes could not be placed with certainty into ... - OPENOpen Access license
A Study on the Phylogeny of the Dyer's Woad Rust Fungus and Other Species of Puccinia from Crucifers
AbstractAbstract
ABSTRACTThe identity of a Puccinia species occurring on the introduced weed dyer's woad (Isatis tinctoria) was studied using sequences from the internal transcribed spacer of the nuclear ribosomal DNA. The relationship of this fungus to other Puccinia ... - OPENOpen Access license
Application of Amplified Restriction Fragment Length Polymorphism for Genetic Characterization of Colletotrichum Pathogens of Alfalfa
AbstractAbstract
ABSTRACTAmplified restriction fragment length polymorphism (AFLP) was used to assess the levels of genomic variations among species and isolates of the genus Colletotrichum. Our objective was to characterize at the molecular level two alfalfa pathogens, ... - OPENOpen Access license
Assessment of Host-Induced Selection on Three Geographic Isolates of Heterodera schachtii Using RAPD and AFLP Markers
AbstractAbstract
ABSTRACTThe hypothesis that host plants exert selection pressure on Heterodera schachtii populations was tested. Host selection of genotypes from three genetically distinct isolates of H. schachtii was assessed using cabbage, sugar beet, oilseed radish (... - OPENOpen Access license
Genetic Variation Among Natural Populations of Tilletia controversa and T. bromi
AbstractAbstract
ABSTRACTIsolates of Tilletia controversa and T. bromi were sampled from wheat and two Bromus species hosts, respectively, in the Pacific Northwest, and genetic variation within and among populations was determined. Fifty-one random amplified polymorphic ... - OPENOpen Access license
Diversity of Sooty Blotch and Flyspeck Fungi on Cider Apples in Spain
AbstractAbstract
Fungi in the sooty blotch and flyspeck (SBFS) complex blemish fruit and reduce the market value of fresh-market apples. In 2010, apples were collected from 16 orchards in northern Spain that had received few to no fungicide sprays. SBFS colonies with the ...